Plasmid_Backbone
Part:BBa_K563053
Designed by: Guo Jing Group: iGEM11_Tianjin (2011-10-05)
vector pYE, designed for inducible expression of recombinant proteins in S.cerevisivae.
pYES2 is a 5.9 kb vector for inducible expression of recombinant proteins in S.cerevisivae. Feature of the vector allow easy cloning of your gene of interest and selection of transformants by uracil prototrophy. The vector contains the follow elements:
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 550
Illegal XbaI site found at 595
Illegal SpeI site found at 525
Illegal SpeI site found at 5851
Illegal PstI site found at 555
Illegal PstI site found at 3632
Illegal PstI site found at 4120 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 550
Illegal NheI site found at 3839
Illegal SpeI site found at 525
Illegal SpeI site found at 5851
Illegal PstI site found at 555
Illegal PstI site found at 3632
Illegal PstI site found at 4120
Illegal NotI site found at 576 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 550
Illegal BamHI site found at 519
Illegal XhoI site found at 583 - 23INCOMPATIBLE WITH RFC[23]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 550
Illegal XbaI site found at 595
Illegal SpeI site found at 525
Illegal SpeI site found at 5851
Illegal PstI site found at 555
Illegal PstI site found at 3632
Illegal PstI site found at 4120 - 25INCOMPATIBLE WITH RFC[25]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 550
Illegal XbaI site found at 595
Illegal SpeI site found at 525
Illegal SpeI site found at 5851
Illegal PstI site found at 555
Illegal PstI site found at 3632
Illegal PstI site found at 4120
Illegal NgoMIV site found at 5709
Illegal AgeI site found at 71 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI.rc site found at 1996
Illegal BsaI.rc site found at 2942
Illegal SapI site found at 913
Illegal SapI site found at 3094
[edit]
Categories
Parameters
n/a | vector pYE, designed for inducible expression of recombinant proteins in S.cerevisivae. |